Biológia das células-tronco mesenquimais de felinos obtidas a partir de nichos presentes no tecido adiposo objetivando sua aplicação terapêutica na medicina veterinária
DOI:
https://doi.org/10.21674/2448-0479.43.368-379Palavras-chave:
fontes, diferenciação, gato, proliferação, terapia celularResumo
O tecido adiposo vêm representando uma fonte em potencial para a obtenção de células-tronco mesenquimais (CTMs). Estudos recentes têm demonstrado que as CTMs apresentam potencial de utilização tanto na pesquisa básica como aplicada. Podendo ser obtidas, em grandes quantidades, por meio de anestesia local e com o mínimo de desconforto, as CTMs vêm sendo objeto de intensa pesquisa. Neste estudo, o tecido adiposo de felino, obtido por meio de biópsia realizada na região subcutânea, foi processado de forma a obter uma população celular morfologicamente homogênea. As células-tronco mesenquimais derivadas do tecido adiposo felinos (CTMs-TAF) foram mantidas, in vitro, em crescimento exponencial até a 9ª passagem apresentando-se como uma população estável e com baixos níveis de senescência. As CTMs-TAF foram capazes de se diferenciarem, in vitro em células adipogênicas, condrogênicas e osteogênicas na presença de factores de indução linhagem específica. Quando injectado em camundongos nude, as CTMs-TAF não foram capazes de dar origem a teratocarcinomas. Estes resultados demonstram de que as CTMs-TAF possuem propriedades que sugerem a sua possível utilização na terapia celular.
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ALOUDI, N.M.; EL ASHIRY, E.A.; FARSI, N.M. Treatment of oral ulcers in dogs using adipose tissue-derived mesenchymal stem cells. The Journal of Clinical Pediatric Dentistry, Birmingham, Alabama, v.38, n.3, p.215-22, 2014.
ALY, L. A.; EL-MENOUFY, H.; SADEQ, H.S. Efficiency of systemic versus intralesional bone marrow-derived stem cells in regeneration of oral mucosa after induction of formocresol induced ulcers in dogs. Dentistry Research Journal, Isfahan, v.11, n.2, p.212-21, 2014.
ARZI, B. et al. Therapeutic Efficacy of Fresh, Autologous Mesenchymal Stem Cells for Severe Refractory Gingivostomatitis in Cats. Stem Cells Translational Medicine, Durham, Carolina do Norte, v.5, n.1, p.75-86. 2016
ARZI, B. et al. Therapeutic Efficacy of Fresh, Allogeneic Mesenchymal Stem Cells for Severe Refractory Feline Chronic Gingivostomatitis. Stem Cells Translational Medicine, Durham, Carolina do Norte, v.6, n.8, p.1710-1722, 2017.
ASSIS, T.L.S.; WINCK, C.P.; SANTOS, E.J.C.S. Análise da Viabilidade Terapêutica das Células-Tronco Mesenquimais Alogênicas no Tratamento de Felino Acometido por Complexo Gengivite Estomatite Felina. Revista Científica Multidisciplinar Núcleo do Conhecimento, São Paulo, Ano 02, v. 1, p.470-482, 2017.
BINATO, R.; SOUZA , F.T.; LAZZAROTTO-SILVA, C. Stability of human mesenchymal stem cells during in vitro culture: considerations for cell therapy. Cell Proliferation, Oxford, v.46, n.1, p.10-22, 2013.
BRITO, H. F. V. et al. Tratamento de sequelas neurológicas em cães, causadas por infecção pelo vírus da cinomose, através do transplante alogênico de células mononucleares de medula óssea. Revista Científica de Medicina Veterinária. Pequenos Animais e Animais de Estimação, Curitiba, v.24, n.8, p.26-29, 2010.
CAPLAN, A.I.; SORRELL, J.M. The MSC curtain that stops the immune system. Immunology Letter, Amsterdam, v.168, n.2, p.136-9, 2015a.
CAPLAN A.I.; HARIRI R. Body Management: Mesenchymal Stem Cells Control the Internal Regenerator. Stem Cells Translational Medicine, Durham, Carolina do Norte, v.4, n.7, p.695-701, 2015b.
CHOI, S. A. et al. Isolation of canine mesenchymal stem cells from amniotic fluid and differentiation into hepatocyte-like cells. In Vitro Cell Developmental Biology Animal, Columbia, Maryland, Jan, v. 49, n. 1, p. 42-51, 2013.
CUERVO, B. et al. Hip osteoarthritis in dogs: a randomized study using mesenchymal stem cells from adipose tissue and plasma rich in growth factors. International journal of molecular sciences, Basel, v.15, n.8, p.13437-60, 2014.
DENNIS, J.E.; CAPLAN, A.I. Differentiation potential of conditionally immortalized mesenchymal progenitor cells from adult marrow of a H-2Kb-tsA58 transgenic mouse. Journal Cell Physiology, Filadelfia, v.167, n.3, p.523–538, 1996.
DENNIS, J.E.; MERRIAM, A.; AWADALLAH, A. A quadric potential mesenchymal progenitor cell isolated from the marrow of an adult mouse. Journal Bone Miner Research, Nova York, v.14, n.5, p.700–709, 1999.
DIMARINO, A.M.; CAPLAN, A.I.; BONFIELD, T.L. Mesenchymal stem cells in tissue repair. Frontiers Immunology, Lusiana, v.4, p.201, 2013.
FILIOLI, U.M.; VALENTINI, L.; LANGE-CONSIGLIO, A. et. al. Isolation, proliferation, cytogenetic, and molecular characterization and in vitro differentiation potency of canine stem cells from foetal adnexa: a comparative study of amniotic fluid, amnion, and umbilical cord matrix. Molecular Reproduction Development, Nova York, v.78, n.5, p.361-373, 2011.
FRIMBERGER, A. E.; MOORE, A. S.; RASSNICK, K. M. A combination chemotherapy protocol with dose intensification and autologous bone marrow transplant (VELCAP-HDC) for canine lymphoma. Journal Veterinary International Medicine, Filadelfia, v.20, n,2, p.355-64, 2006.
GATTI A; MARTINS, D.S.; SANTOS, E.J.C. Cell therapy in the treatment of myeloid aplasia - a case report. Revista Científica de Medicina Veterinária – Pequenos Animais e Animais de Estimação, São Paulo, v.12, n.41, p.296-303, 2014.
GEBUREK, F. et al. Effect of single intralesional treatment of surgically induced equine superficial digital flexor tendoncore lesions with adipose-derived mesenchymal stromal cells: a controlled experimental trial. Stem Cell Research Therapy, Londres, v.8, n.1, p.129, 2017.
HARMAN, R. et al. A Prospective, Randomized, Masked, and Placebo-Controlled Efficacy Study of Intraarticular Allogeneic Adipose Stem Cells for the Treatment of Osteoarthritis in Dogs. Frontiers Veterinary in Science, Lusiana, v.3, 2016.
JOHNSTONE, B.; HERING, T.M.; CAPLAN, A.I. In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells. Experimental Cell Research, Nova York, v.238, n.1, p.265–272, 1998.
JONES, E.A.; KINSEY, S.E.; ENGLISH, A. Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells. Arthritis Rheumatology Journal, Atlanta, v.46, n.12, p,3349-3360, 2002.
KFOURY, Y.; SCADDEN, D.T. Mesenchymal cell contributions to the stem cell niche. Cell Stem Cell, Cambridge, v.16, n.3, p.239-253, 2015.
KADIYALA, S.; YOUNG, R.G.; THIEDE, M.A. Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro. Cell Transplantation, Elmsford, Nova York, v.6, n.2, p.125-134, 1997.
KIM, J.W.; LEE, J.H.; LYOO, Y.S. The effects of topical mesenchymal stem cell transplantation in canine experimental cutaneous wounds. Veterinary Dermatology, Oxford, v.24, n.2, p.242-253, 2013.
KIM, Y. et al. Antioxidant and anti-inflammatory effects of intravenously injected adipose derived mesenchymal stem cells in dogs with acute spinal cord injury. Stem Cell Research Therapy, Londres, v.26, n.6, p.229, 2015.
KIM, Y. et al. Transplantation of adipose derived mesenchymal stem cells for acute thoracolumbar disc disease with no deep pain perception in dogs. Journal of Veterinary Science, Seul, v.17, n.1, p.123-126, 2016.
KISIEL, A.H.; MCDUFFEE, L.A.; MASAOUD, E. Isolation, characterization, and in vitro proliferation of canine mesenchymal stem cells derived from bone marrow, adipose tissue, muscle, and periosteum. American Journal of Veterinary Research, Chicago, v.73, n.8, p.1305-1317, 2012.
KOCH, T.G.; HEERKENS, T.; THOMSEN, P.D. Isolation of mesenchymal stem cells from equine umbilical cord blood. BMC Biotechnology, Londres, v.7, p.26, 2007.
KRISTON-PÁL, É. et al. Characterization and therapeutic application of canine adipose mesenchymal stem cells to treat elbow osteoarthritis. Canadian Journal of Veterinary Research, Ottawa, v.81, n.1, p.73-78, 2017.
LEE, S.Y. et al. Treatment of Lateral Epicondylosis by Using Allogeneic Adipose-Derived Mesenchymal Stem Cells: A Pilot Study. Stem Cells, Durham, Carolina do Norte, v.33, n.10, p.2995-3005, 2015.
MARTIN, D. R.; COX, N.R.; HATHCOCK, T.L. Isolation and characterization of multipotential mesenchymal stem cells from feline bone marrow. Experimental Hematology, Copenhague, v.30, n.8, p.879-886, 2002.
MEIRELLES, L.D.A.S.; NARDI, N.B. Murine marrow-derived mesenchymal stem cell: isolation, in vitro expansion, and characterization. British Journal of Haematology, Oxford, v.123, n.4, p.702-711, 2003.
MOLL, G.; RASMUSSON-DUPREZ, I.; VON BAHR, L. Are therapeutic human mesenchymal stromal cells compatible with human blood? Stem Cells, Durham, Carolina do Norte, v.30, n.7, p.1565-1574, 2012.
MORIYAMA, H.; KASASHIMA, Y.; KUWANO, A. Anatomical location and culture of equine corneal epithelial stem cells. Veterinary Ophthalmology, Osney Mead, Oxford, v.17, p.106-112, 2014.
MURPHY, M.B.; MONCIVAIS, K.; CAPLAN, A.I. Mesenchymal stem cells: environmentally responsive therapeutics for regenerative medicine. Experimental Molecular Medicine, Seul, Nov 15, p.45-54, 2013.
NEUPANE, M.; CHANG, C.C.; KIUPEL, M.Y. Isolation and characterization of canine adipose-derived mesenchymal stem cells. Tissue Engineering Part A, New Rochelle, Nova York , v.14, n.6, p.1007-1015, 2008.
PENHA, E. M.; MEIRA, C.S.; GUIMARÃES, E.T. Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs. Stem Cells International, Londres, 2014.
REICH, C.M.; RAABE, O.; WENISCH, S. Isolation, culture and chondrogenic differentiation of canine adipose tissue- and bone marrow-derived mesenchymal stem cells--a comparative study. Veterinary research communications, Amsterdam, v.36, n.2, p.139-148, 2012.
RENTSCH, C.; HESS, R.; RENTSCH, B. Ovine bone marrow mesenchymal stem cells: isolation and characterization of the cells and their osteogenic differentiation potential on embroidered and surface-modified polycaprolactone-co-lactide scaffolds. In Vitro Cell Development Biology Animal, Columbia, Maryland, v.46, n.7, p.624-634, 2010.
RINGE, J.; KAPS, C.; SCHMITT, B. Porcine mesenchymal stem cells. Induction of distinct mesenchymal cell lineages. Cell Tissue Research, Berlim, v.307, n.3, p.321-327, 2002.
SANTOS, E.J.C.; WINCK, C.P.; BRAGA, C.L. Estudo Eficácia E Segurança Terapêutica Das Células-Tronco Mesenquimais Alogênicas No Tratamento de Felinos Acometidos pela Doença Renal Crônica e Resistentes a Eritropoetina Sintética. Revista Científica Multidisciplinar Núcleo do Conhecimento, São Paulo, Ano 02, v.1. p. 296-309, 2017a.
SANTOS, E.J.C. Análise da Aplicação Terapêutica das Células Tronco na Medicina Veterinária. Revista Científica Multidisciplinar Núcleo do Conhecimento, São Paulo, Ano 02, v. 1, p. 269-295, 2017b.
SATO, K. Isolation and characterisation of peripheral blood-derived feline mesenchymal stem cells. Veterinary Journal, Londres, v.216, p.183-8, 2016.
SEO, M.S.; JEONG, Y.H.; PARK, J.R. Isolation and characterization of canine umbilical cord blood-derived mesenchymal. Stem cells, Seul, v.10, n.3, p.181-187, 2009.
TOMA, C.; PITTENGER, M.F.; CAHILL, K.S. Human mesenchymal stem cells differe- ntiate to a cardiomyocyte phenotype in the adult murine heart. Circulation, Dallas, v.105, p.93–98, 2002.
TRZIL, J.E.; MASSEAU, I.; WEBB, T.L. Intravenous adipose-derived mesenchymal stem cell therapy for the treatment of feline asthma: a pilot study. Journal of Feline Medicine and Surgery, Londres, Sep 17, 2015.
TSIMBOURI, P. M. Adult Stem Cell Responses to Nanostimuli. Journal of Functional Biomaterials, Basel, v.6, n.3, p.598-622, 2015.
VIDAL, M.A. et al. Characterization of equine adipose tissue-derived stromal cells: adipogenic and osteogenic capacity and comparison with bone marrow-derived mesenchymal stromal cells. Veterinary Surgery, Filadelfia, v.36, n.7, p.613-22, 2007.
WEI, H.; OOI, T.H.; TAN, G. Cell delivery and tracking in post-myocardial infarction cardiac stem cell therapy: an introduction for clinical researchers. Heart Fail Review, Norwell, v.15, n.1, p.11-14, 2010.
WINCK, C.P. Cultivo e caracterização de células-tronco de membrana amniótica canina. 2009. Trabalho de Conclusão de Curso - Graduação em Ciências Biologias, Universidade de Santo Amaro, São Paulo, 2009.
WORSTER, A. A.; NIXON, A. J.; BROWER-TOLAND, B. D. Williams J. Effect of transforming growth factor beta1 on chondrogenic differentiation of cultured equine mesenchymal stem cells. American Journal of Veterinary Research, Chicago, v.61, n.9, p.1003-1010, 2000.
XU, Y. Q.; LIU, Z. C. Therapeutic potential of adult bone marrow stem cells in liver disease and delivery approaches. Stem Cell Revew, Totowa, v.4, n.2, p.101-112, 2008.
ZUK, P. A. et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Engineering, Nova York, v.7, n.2, p.211-228, 2001.
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